BrdU anticorps (FITC)
Aperçu rapide pour BrdU anticorps (FITC) (ABIN336862)
Antigène
Voir toutes BrdU AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Specificité
- Bromodeoxyuridine.
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Immunogène
- BrdU conjugated to KLH
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Isotype
- IgG1 kappa
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Indications d'application
- IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears) For IHC dilute conc. antibodies 1:50-1:100, use streptavidin-biotin system or polymer system, incubate 30 minutes at room temperature. Prediluted antibody is ready to be used for IHC. (For staining of FFPE tissue sections, incubate with 4 N HCl for 20 minutes at RT, followed by treatment with proteolytic digestion for 10 minutes at RT. (Please refer to our Trypsin (AR-6541), Pronase (AR-6542) or pepsin (AR-6543) data sheets. IF, FITC-conjugated antibodies 10-20 ug/ml (1:10-1:20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4ºC. Flow: FITC-conjugated antibodies, 0.2-1.0 ug/0.1 ml (1:200-1:1,000) (Not tested in our lab). The optimum dilution should be determined by the individual lab. Positive Control: Liver of experimental animal injected with BrdU or cell line grown in presence of BrdU. Cellular Localization: Nuclear.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- The concentrated antibodies are supplied in PBS with 1% BSA, 0.05% azide, pH 7.4. The Prediluted antibody is supplied in our Universal antibody dilution buffer (AR-6526) green in color. Description Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a syn
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C
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- BrdU (Bromodeoxyuridine (BrdU))
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Autre désignation
- BRDU
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Classe de substances
- Chemical
Antigène
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